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Therefore, we evaluated the influence of two different post-collection temperatures (20 ☌ and 37 ☌) and various examination time points (3–30 min) after semen collection on semen quality. The aim of this study was to investigate two possible factors affecting buck sperm quality after collection prior to adding an extender. Although different studies have evaluated the influence of temperature on semen quality in mammal species systematic investigations on caprine semen in this critical post-collection period prior to adding an extender are still missing. Īt present, however, only a limited number of studies have assessed the factors that can influence the semen analysis results of raw goat semen. Deviations may be due to accessory gland disease and can have an adverse effect on semen viability. In ruminants pH of semen is in the slightly acidic with range of 6.4–7.0.
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In general, semen is usually preserved at 18–22 ☌ or at 37 ☌ until adding an extender. In literature different handling temperatures for goat spermatozoa after collection are described varying from 30 ☌ to 37 ☌. Sperm motility was better for undiluted semen samples stored at 15 ☌ and 20 ☌ for 48 h compared to 4 ☌ and 39 ☌. It is known that boar spermatozoa are very susceptible to cold shock, especially when stored lower than 15 ☌. Equine semen should be kept at 37 ☌ prior to dilution. The sperm of mammals are very sensitive to temperature fluctuations with species and individual differences. All materials having contact with the sperm should be preheated to this temperature. The handling temperature during semen evaluation is usually 37 ☌.
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Busch and Fischer (2007) suggested that the collected semen must be evaluated within 10 min after collection, without describing the influence of time on semen quality. In other studies the time interval between collection and evaluation was 2–3 min, after 20 min or for the entire evaluation within one hour after collection, while concentration, pH and volume were examined immediately after collection. Usually the semen evaluation takes place directly after the collection.
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Ī standard protocol for evaluation of goat semen does not exist. In human medicine, guidelines for semen collection, handling and evaluation of the ejaculates are described. Therefore, the handling conditions must be kept as constant as possible during the evaluation. These are used to determine the suitability of an ejaculate for further use and to establish an insemination dose. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.ĭifferent parameters are assessed as part of the semen analysis including in general volume, color, consistency, impurities as well as semen concentration, motility, vitality and morphology. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. ConclusionĮxamination time point was identified as factor strongly influencing raw peacock buck semen after collection. ResultsĮxamination time point had a significant influence on raw sperm viability ( p 0.05), motility ( p > 0.05), with the exception of fast moving sperm ( p = 0.04), or on semen pH ( p > 0.05). Here we examined the effects of two handling temperatures (20 ☌, 37 ☌) and various examination time points (3–30 min) after semen collection. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing.